Techniques Series: Next Generation Sequencing technologies

May 31, 2013 | Posted by Guest in Research |
Transcriptome SOLiD sequencer by EMSL, on Flickr
Creative Commons Attribution-Noncommercial-Share Alike 2.0 Generic License  by  EMSL 

 

This is a guest post by James Hadfield, Head of the Genomics Core Facility at Cancer Research UK.

Today there are three main next-generation sequencing (NGS) technologies; Illumina, Ion Torrent and 454. Sanger sequencing is still used by almost all research labs and remains a key tool for simple clone verification or PCR based sequencing.

Although the DNA sequencing in each system is very different, all three technologies share many commonalities; they generally start with fragmented genomic DNA to which oligonucleotide adapters are ligated, and single adapter-ligated molecules are clonally amplified ready for highly-parallel sequencing of millions or even billions of reads. The technologies were conceived and developed as the Human Genome Project (HGP) was finishing. Sanger sequencing was a brute force tool; requiring an international effort, billions of dollars and 15 years to complete the single HGP genome.

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SEASR Conference for Core Facilities

May 28, 2013 | Posted by Team in Conferences |

seasr-logo-web

The Southeastern Association of Shared Resources (SEASR), a network of university core and shared resource facilities, will be hosting its launch conference this June, with support from the Science Exchange community.

The conference will be the first annual meeting hosted by the SEASR association, helping to provide a forum for core facility directors and scientists to discuss best practices and advances in technological applications. Science Exchange, in its continuing support for core facilities and the work they do to help scientists access high-end equipment at low-cost rates, is proud to be an initial corporate sponsor of the event, and will be hosting a workshop at the conference.

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Solving The Research Integrity Crisis

May 20, 2013 | Posted by Elizabeth in Research |

Earlier this month, I had the pleasure of attending the third World Conference on Research Integrity in Montreal, bringing together thought leaders on research integrity and responsible conduct in research. The Conference covered issues including the contributing factors of fabrication and systemic dishonesty, potential solutions in better training and support for whistleblowers, and larger incentives to changing the research culture.

Aggregating these respective themes, I felt it important to review the different opinions offered at the Conference. Consolidating the various themes and propositions presented can in turn allow for discussion of potential strategies to build more effective solutions to the problem of research integrity.

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10 Free Scientific Resources For Graduating Students

May 16, 2013 | Posted by Piper in Research |
Open_Access_PLoS.svg by PGRsOnline, on Flickr
Creative Commons Attribution-Noncommercial-Share Alike 2.0 Generic License  by  PGRsOnline 

 

Upon finishing my Ph.D. in December, I was quickly confronted with the loss of journal, publication, and general program access.

Within an academic institution, we are privileged in access to a wide array of resources and traditionally subscription-based service. And while there are far more open-access resources than ever before, with Wikipedia and PLOS as significant examples, it is important to recognize some of the other commonly available resources which can assist scientists who are set to graduate this summer from their institutions.

Below I highlight some of the references, software, and literature I myself am using that are all free, open access, and ready to use. And as the discussion about open access scientific literature makes significant strides, I think it is important to start thinking about what other resources and expertise should be available for scientists to freely access and use.

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Techniques Series: Using Flow Cytometry

May 14, 2013 | Posted by Roshan in Research |
Work2 by adpal3180, on Flickr
Creative Commons Attribution-Noncommercial-No Derivative Works 2.0 Generic License  by  adpal3180 

 

Flow Cytometry is a powerful technique that enables researchers to conduct a rapid and multi-parametric analysis of single cells simultaneously.

Of late, it has become an indispensable tool in basic cell biology and medical research, immunological studies, drug discovery, and even diagnosis of diseases. The applications vary as well, from cell sorting of heterogeneous populations, DNA abnormalities, apoptosis assays, cell cycle analyses, immunophenotyping, protein modifications, proliferation assays, and cell signaling.

Below is a broad overview of the technique platform, how it works in these applications, and new technological developments.

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Fermentation & Protein Purification Workshop

May 1, 2013 | Posted by Team in Workshops |

fermentor

The University of Maryland’s Biotechnology Research and Education Program (BREP) is offering two workshops to help biotechnology professionals advance their careers and learn the latest technologies.

With the event hosted by the directors of the Bioprocess Scale-Up Facility and Biopharmaceutical Advancement Facility (one of the top providers on Science Exchange), you can come to learn how to produce and purify biopharmaceutical products.  The hands-on, one-week training includes two sessions taught by world-class faculty and practitioners.  Academic and government discounts are available as well.

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