Reproducibility

Reproducibility

The Reproducibility Project: Cancer Biology – Experiments have begun

The RPCB is a first of its kind attempt to directly replicate a subset of high-impact, pre-clinical cancer biology papers. Importantly, the methodology, quality control steps and replication data will be open and accessible on the Open Science Framework.

We are very excited to report that 13 Registered Reports have been accepted in eLife, and experiments from 12 of those studies are underway. These include:

  • Registered Report: BET bromodomain inhibition as a therapeutic strategy to target c-Myc
  • Registered Report: Interactions between cancer stem cells and their niche govern metastatic colonization
  • Registered Report: Coadministration of a tumor-penetrating peptide enhances the efficacy of cancer drugs
  • Registered Report: Discovery of preclinical validation of drug indications using compendia of public gene expression data
  • Registered Report: Intestinal inflammation targets cancer-inducing activity of the microbiota
  • Registered Report: Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells
  • Registered Report: The CD47-signal regulatory protein alpha (SIRPa) interaction is a therapeutic target for human solid tumors
  • Registered Report: Transcriptional amplification in tumor cells with elevated c-Myc
  • Registered Report: Senescence surveillance of pre-malignant hepatocytes limits liver cancer development
  • Registered Report: Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors
  • Registered Report: Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretion
  • Registered Report: Melanoma genome sequencing reveals frequent PREX2 mutations

Before each replicating lab begins experimental work, critical reagents (often kindly shared by authors of the original studies) are quality checked. For example, all of the cell lines are authenticated and mycoplasma tested, plasmid sequences are sequenced, and rodents are pathogen tested. These quality check steps will be included on the Open Science Framework along with the data for the replication experiments themselves.

 

Tracking Our Progress

Keep track here as we continue to move projects forward. Our current status as of July 2015 is described below:

Reproducibility project progress

Phase:

  1. Replication experiments identified for each original paper
  2. Protocols drafted
  3. Protocols transferred to Registered Report format
  4. Review and feedback from original authors (requests for necessary reagents)
  5. Expert provider identified
  6. Registered Report peer reviewed at eLife
  7. Experimental work
  8. Experiment work is finished
  9. Replication experiments analyzed and evaluated
  10. Replication Study published in eLife
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